Real-time PCR (also called quantitative PCR or qPCR) detects accumulation of amplicon during the reaction, instead of detecting PCR products after the reaction. When detecting reaction products at an exponential phase of their accumulation there is a quantitative relationship between amount of starting template and amount of PCR product at given cycle. Real-time PCR therefore allows quantification of DNA easier and more precise than end-point PCR.

Table. Instrument compatibility.

Product	Applied Biosystems		Bio-Rad	Stratagene	Roche	Corbett	Eppendorf	Cepheid
Product	7300, 7900HT, ABI PRISM 7000, ABI PRISM 7700, StepOne Plus, StepOne	7500	iCycler iQ, My iQ, iQ5	Mx4000, Mx3005P, Mx3000P	LightCycler 480, LightCycler 2	Rotor-Gene 3000, Rotor-Gene 6000	MasteCycler ep realplex	SmartCycler
Maxima® SYBR Green/ROX qPCR Master Mix (2X)	***	*	*	*	*	*	*	*
Maxima® SYBR Green/Fluorescein qPCR Master Mix (2X)			***
Maxima® SYBR Green qPCR Master Mix (2X), ROX Solution provided	*	***	*	***	***	***	***	***
Maxima® Probe/ROX qPCR Master Mix (2X)	***	*	*	*	*	*	*	*
Maxima® Probe qPCR Master Mix (2X), ROX Solution provided	*	***	***	***	***	***	***	***

*– suitable.
*** – best.

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Products » All » PCR, qPCR, RT-PCR & dNTPs » Real-time PCR » Maxima® SYBR Green qPCR Master Mix (2X), ROX Solution provided

Real-time PCR

Maxima® SYBR Green qPCR Master Mix (2X), ROX Solution provided

- Store at -20°C

Catalog#	Size, concentration	Supplied with:		Certificate of Analysis	MSDS
Catalog#	Size, concentration	Water, nuclease-free	ROX Solution (50 µM)	Certificate of Analysis	MSDS
K0251	2 x 1.25 ml (for 200 react. of 25 µl)	2 x 1.25 ml	50.00 µl	K0251
K0252	10 x 1.25 ml (for 1000 react. of 25 µl)	10 x 1.25 ml	250.00 µl	K0252
K0253	4 x 12.5 ml (for 4000 react. of 25 µl)	2 x 30.00 ml	1.00 ml	K0253

Product information

Features

Specificity – Maxima® Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers.
Sensitivity – detects low copy number targets.
ROX Solution provided in a separate tube.
Wide linear range – accurate quantification across 9 orders of magnitude.
Universal – can be used on most real-time PCR instruments.
Reproducibility and convenience – ready-to-use 2X master mix.

Applications

Real-time PCR and RT-qPCR using SYBR® Green dye on most real-time PCR machines.

Description

Maxima® SYBR Green qPCR Master Mix (2X) is a ready-to-use solution optimized for quantitative real-time PCR and two-step real-time RT-PCR on most real-time PCR machines. The master mix includes Maxima® Hot Start Taq DNA polymerase, SYBR® Green I dye and dNTPs in an optimized PCR buffer. ROX Solution is provided in a separate vial for use with machines that require ROX as a passive reference dye. Maxima® Hot Start Taq DNA polymerase in combination with the optimized buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima® SYBR Green qPCR Master Mix in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Quality Control

The absence of endo-, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests. Functional tests in parallel 25 μl real-time PCR reactions containing 10-fold dilutions of human genomic DNA demonstrate a linear resolution over five orders of magnitude.

Notice

Use of UDG for PCR carryover decontamination is covered by patents in certain countries and may require a license.

Figure 1. Uniform and reproducible results with two different real-time PCR instruments.
Amplification of the human cMyc gene was performed on serial dilutions of Jurkat cell total RNA (10 ng to 100 fg). First strand cDNA was synthesized with RevertAid™ Premium Reverse Transcriptase (#EP0731). The resulting cDNA was amplified using the Maxima® SYBR Green qPCR Master Mix (2X) in duplicate reactions. Reactions were performed on ABI 7500 (A) and Corbett RotorGene™ 3000 (B). The amplification plot and the melting curve show high sensitivity and high specificity of the amplification.

Patents, Licenses, Trademarks

Protocols & recommendations

RECOMMENDATIONS FOR USE

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