Real-time PCR (also called quantitative PCR or qPCR) detects accumulation of amplicon during the reaction, instead of detecting PCR products after the reaction. When detecting reaction products at an exponential phase of their accumulation there is a quantitative relationship between amount of starting template and amount of PCR product at given cycle. Real-time PCR therefore allows quantification of DNA easier and more precise than end-point PCR.

Table. Instrument compatibility.

Product	Applied Biosystems		Bio-Rad	Stratagene	Roche	Corbett	Eppendorf	Cepheid
Product	7300, 7900HT, ABI PRISM 7000, ABI PRISM 7700, StepOne Plus, StepOne	7500	iCycler iQ, My iQ, iQ5	Mx4000, Mx3005P, Mx3000P	LightCycler 480, LightCycler 2	Rotor-Gene 3000, Rotor-Gene 6000	MasteCycler ep realplex	SmartCycler
Maxima® SYBR Green/ROX qPCR Master Mix (2X)	***	*	*	*	*	*	*	*
Maxima® SYBR Green/Fluorescein qPCR Master Mix (2X)			***
Maxima® SYBR Green qPCR Master Mix (2X), ROX Solution provided	*	***	*	***	***	***	***	***
Maxima® Probe/ROX qPCR Master Mix (2X)	***	*	*	*	*	*	*	*
Maxima® Probe qPCR Master Mix (2X), ROX Solution provided	*	***	***	***	***	***	***	***

*– suitable.
*** – best.

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Products » All » PCR, qPCR, RT-PCR & dNTPs » Real-time PCR » Maxima® SYBR Green/Fluorescein qPCR Master Mix (2X)

Real-time PCR

Maxima® SYBR Green/Fluorescein qPCR Master Mix (2X)

- Store at -20°C

Catalog#	Size, concentration	Supplied with:	Certificate of Analysis	MSDS
Catalog#	Size, concentration	Water, nuclease-free	Certificate of Analysis	MSDS
K0241	2 x 1.25 ml (for 200 react. of 25 µl)	2 x 1.25 ml	K0241
K0242	10 x 1.25 ml (for 1000 react. of 25 µl)	10 x 1.25 ml	K0242
K0243	4 x 12.5 ml (for 4000 react. of 25 µl)	2 x 30.00 ml	K0243

Product information

Features

Specificity – Maxima® Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers.
Sensitivity – detects low copy number targets.
Fluorescein – supplemented with fluorescein passive reference dye for use with Bio-Rad iCycler iQ®, MyiQ™ or iQ5™.
Wide linear range – accurate quantification across 9 orders of magnitude.
Reproducibility and convenience – ready-to-use 2X master mix.

Applications

Real-time PCR and RT-qPCR using SYBR® Green dye on Bio-Rad iCycler iQ®, MyiQ™ or iQ5™ machines.

Description

Maxima® SYBR Green/Fluorescein qPCR Master Mix (2X) is a ready-to-use solution optimized for quantitative real-time PCR and two-step real-time RT-PCR with the Bio-Rad iCycler iQ®, MyiQ™ or iQ5™ machines. The master mix includes Maxima® Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. It contains SYBR® Green I dye and is supplemented with fluorescein passive reference dye. Only template and primers need to be added.

Maxima® Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes. The fluorescein included in the master mix allows for the collection of Dynamic Well Factors with the Bio-Rad iCycler iQ®, MyiQ™ or iQ5™ machines, but does not affect qPCR efficiency. dUTP is included in the mix for optional carryover contamination control using uracil DNA glycosylase (UDG).

The use of Maxima® SYBR Green/Fluorescein qPCR Master Mix (2X) in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Quality Control

The absence of endo-, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests. Functional tests in parallel 25 µl real-time PCR reactions containing 10-fold serial dilutions of human genomic DNA demonstrate a linear resolution over five orders of dynamic range.

Notice

Use of UDG for PCR carryover decontamination is covered by patents in certain countries and may require a license.

Figure 1. Uniform and reproducible results.
Amplification of 10-fold serial dilutions of supercoiled plasmid DNA, starting from 10 ng down to 0.1 fg, using the Maxima® SYBR Green/Fluorescein qPCR Master Mix (2X) in duplicate reactions. Reactions were performed on the Bio-Rad iCycler iQ® instrument. The amplification plot shows linearity across 9 orders of magnitude.

Figure 2. Melting curve analysis confirms high qPCR specificity.
Amplification of 10-fold serial dilutions of supercoiled plasmid DNA, starting from 10 ng down to 0.1 fg, using the Maxima® SYBR Green/Fluorescein qPCR Master Mix (2X) in duplicate reactions. Reactions were performed on the Bio-Rad iCycler iQ® instrument. NTC = non-template control.

Patents, Licenses, Trademarks

Protocols & recommendations

RECOMMENDATIONS FOR USE

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