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Nucleotides
Nucleotides,
#P22, 183 KB
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PCR, qPCR, RT-PCR & dNTPs

dNTP Set

  • LO certified - LO certified
Catalog# Size, concentration Certificate of Analysis MSDS
R0181 4 x 0.25 ml (4 x 25 µmol of 100 mM solution) R0181
R0182 4 x 1 ml (4 x 100 µmol of 100 mM solution) R0182
R0186 4 x 5 ml (4 x 500 µmol of 100 mM solution) R0186
Product information
Features

  • Greater than 99% purity confirmed by HPLC.
  • Free of human and E.coli DNA.
  • Highly stable – the neutral pH of nucleotide solutions ensures the stability during long-term storage:
    – stable after multiple freeze-thaw cycles;
    – 90-95% of dNTPs remain in triphosphate form even after 7 weeks at room temperature;
    – 85-90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 min at 94°C; 3 min at 72°C).
  • Application tested in:
    – long range PCR (40 kb),
    – cDNA synthesis and RT-PCR,
    – real-time PCR,
    – standard PCR,
    – high fidelity PCR.
Applications

  • For use in all molecular biology applications including PCR, real-time PCR, high fidelity and long PCR, LAMP-PCR, cDNA synthesis, RT-PCR, RDA, MDA, DNA labeling and DNA sequencing.
Description
PureExtreme® dNTPs are manufactured at Fermentas, one of a few primary manufacturers in the industry. They are supplied in aqueous solutions titrated to pH 7.0 with NaOH. dNTPs are available individually, as a convenient set of 100 mM solutions of each dATP, dTTP, dCTP and dGTP for direct use in PCR, reverse transcription, labeling and other applications.

Quality Control
  • Functionally tested in PCR.
  • Greater than 99% purity of each component confirmed by HPLC.
  • Free of endo-, exodeoxyribonuclease, ribonuclease and nicking activities.

Storage
Store at -20°C.



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FERMENTAS INTERNATIONAL INC
tel: 905 333 8355
fax: 905 333 3135
fermentas.info@thermofisher.com

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