Highly stable – the neutral pH of nucleotide solutions ensures the stability during long-term storage: – stable for years at -20°C; – stable after multiple freeze-thaw cycles; – 90-95% of dNTPs remain in triphosphate form even after 7 weeks at room temperature;
– 85-90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 min at 94°C; 3 min at 72°C).
Application tested in: – long range PCR (40 kb), – cDNA synthesis and RT-PCR, – real-time PCR, – standard PCR, – high fidelity PCR.
Applications
For use in all molecular biology applications including PCR, real-time PCR, high fidelity and long PCR, LAMP-PCR, cDNA synthesis, RT-PCR, RDA, MDA, DNA labeling and DNA sequencing.
Description
PureExtreme® dNTPs are manufactured at Fermentas, one of a few primary manufacturers in the industry. They are supplied in aqueous solutions titrated to pH 7.0 with NaOH. dNTPs are available individually as nucleotide mixes at convenient concentrations for direct use in PCR, reverse transcription, labeling and other applications.
Quality Control
Functionally tested in PCR.
Greater than 99% purity of each component confirmed by HPLC.
Free of endo-, exodeoxyribonuclease, ribonuclease and nicking activities.
- Worldwide
- to print
- LO certified
