Four-times greater accuracy than Taq DNA polymerase.
Long amplification products: – up to 20 kb with viral DNA as template, – up to 10 kb with genomic DNA as template.
High yields.
Robust.
Applications
Generation of PCR products for purposes of cloning, expression, site-directed mutagenesis.
High yield PCR.
Long range PCR.
RT-PCR.
Description
High Fidelity PCR Enzyme Mix is a unique blend of Fermentas Taq DNA Polymerase and a thermostable DNA polymerase with proofreading activity. The two enzymes synergistically generate longer PCR products with greater yield and fidelity than Taq DNA Polymerase alone. Thus, fidelity of the High Fidelity PCR Enzyme Mix exceeds more than four times the fidelity of Taq DNA Polymerase. In addition, the enzyme mix can generate long amplicons up to 20 kb with viral DNA templates and up to 10 kb with genomic DNA templates. Such long amplicons are often difficult to generate with a single DNA polymerase.
Quality Control
Generation of 20 kb and 9 kb amplicons with lambda DNA and human DNA as templates, respectively.
Note
High Fidelity PCR Enzyme Mix generates both blunt-ended and 3'-dA tailed PCR products.
Blunting of PCR product prior to cloning is highly recommended.
For efficient PCR product cloning, use the CloneJET™ PCR Cloning Kit.
Figure 1. Accuracy of thermostable DNA polymerases and DNA polymerase mixes. Accuracy – an average number of nucleotides the polymerase incorporates before making an error. Polymerase accuracy was measured by amplification of a 323 bp lacZ α gene and analysis of the cloned PCR products using the modified method (1).
- Worldwide
- to print
- Store at -20°C
Figure 1. Accuracy of thermostable DNA polymerases and DNA polymerase mixes.
