Hot start PCR uses enzymes that are inactive at room temperature and are activated during the initial denaturation step of PCR. Such polymerases avoid extension of non-specifically annealed primers or primer dimmers during the reaction set-up. Use of hot start DNA polymerases enhances the specificity, sensitivity and yield of DNA amplification. In addition, it provides the convenience of room temperature reaction set-up.

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Products » All » PCR, qPCR, RT-PCR & dNTPs » Hot Start PCR » Maxima® Hot Start Green PCR Master Mix (2X)

Hot Start PCR

Maxima® Hot Start Green PCR Master Mix (2X)

- Store at -20°C

Catalog#	Size, concentration	Supplied with:	Certificate of Analysis	MSDS
Catalog#	Size, concentration	Water, nuclease-free	Certificate of Analysis	MSDS
K1061	2 x 1.25 ml (for 100 react. of 50 µl)	2 x 1.25 ml	K1061
K1062	10 x 1.25 ml (for 500 react. of 50 µl)	10 x 1.25 ml	K1062

Product information

Features

Convenient – Maxima® Hot Start Taq DNA Polymerase in a ready-to-use mix.
Hot start PCR – reaction set-up at room temperature.
Four minute activation time.
Direct loading of PCR product on gels.
Highs specificity and sensitivity of PCR.

Applications

High throughput hot start PCR.
RT-PCR.
Highly specific amplification of complex genomic and cDNA templates.
Amplification of low copy DNA targets.
Generation of PCR products for TA cloning.

Description

Maxima® Hot Start Green PCR Master Mix (2X) is a ready-to-use solution containing Maxima® Hot Start Taq DNA Polymerase, optimized hot start PCR buffer, Mg²⁺, and dNTPs. The master mix is supplemented with two tracking dyes and a density reagent that allows for direct loading of PCR products on a gel. The dyes in the master mix do not interfere with PCR performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion. The master mix retains all features of Maxima® Hot Start Taq DNA Polymerase.
It is capable of amplification of up to 3 kb from genomic DNA. For applications that require PCR product analysis by absorbance or fluorescence excitation, we recommend using the colorless Maxima® Hot Start PCR Master Mix (2X) (#K0151).

Quality Control

The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests. Functionally tested in hot start PCR.

Storage

Store at -20°C.

Figure 1. Stability of Maxima® Hot Start Green PCR Master Mix (2X).
Amplification of 956 bp single copy gene from 0.1 µg human genomic DNA using PCR Master Mix (2X) in 50 µl reaction mixture.
M – GeneRuler™ Express DNA Ladder (#SM1551)
1,2 – PCR product generated with 2 u of Maxima® Hot Start Taq DNA Polymerase
3, 4 – PCR product generated with Maxima® Hot Start Green PCR Master Mix (2X) stored at -20°C
5, 6 – PCR product generated with Maxima® Hot Start Green PCR Master Mix (2X) after 25 freeze-thaw cycles

Figure 2. 1X reaction mixture containing Maxima® Hot Start Green PCR Master Mix.
A – unseparated in a well
B – blue and yellow dyes after separation.

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Protocols & recommendations

RECOMMENDATIONS FOR USE

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