Hot start PCR uses enzymes that are inactive at room temperature and are activated during the initial denaturation step of PCR. Such polymerases avoid extension of non-specifically annealed primers or primer dimmers during the reaction set-up. Use of hot start DNA polymerases enhances the specificity, sensitivity and yield of DNA amplification. In addition, it provides the convenience of room temperature reaction set-up.

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Products » All » PCR, qPCR, RT-PCR & dNTPs » Hot Start PCR » Maxima® Hot Start PCR Master Mix (2X)

Hot Start PCR

Maxima® Hot Start PCR Master Mix (2X)

- Store at -20°C

Catalog#	Size, concentration	Supplied with:	Certificate of Analysis	MSDS
Catalog#	Size, concentration	Water, nuclease-free	Certificate of Analysis	MSDS
K1051	2 x 1.25 ml (for 100 react. of 50 µl)	2 x 1.25 ml	K1051
K1052	10 x 1.25 ml (for 500 react. of 50 µl)	10 x 1.25 ml	K1052

Product information

Features

Convenient – Maxima® Hot Start Taq DNA Polymerase in a ready-to-use mix.
Hot start PCR – reaction set-up at room temperature.
Four minute activation time.
High specificity and sensitivity of PCR.

Applications

High throughput hot start PCR.
RT-PCR.
Highly specific amplification of complex genomic and cDNA templates.
Amplification of low copy DNA targets.
Generation of PCR products for TA cloning.

Description

Maxima® Hot Start PCR Master Mix (2X) is a ready-to-use solution containing Maxima® Hot Start Taq DNA Polymerase, optimized hot start PCR buffer, Mg²⁺, and dNTPs. The master mix retains all features of Maxima® Hot Start Taq DNA Polymerase. It is capable of amplification of up to 3 kb from genomic DNA. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible hot start PCR.

Quality Control

The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests. Functionally tested in hot start PCR.

Storage

Store at -20°C.

Figure 1. Stability of Maxima® Hot Start PCR Master Mix (2X).
Amplification of 956 bp single copy gene from 0.1 µg human genomic DNA using PCR Master Mix (2X) in 50 µl reaction mixture.
M – GeneRuler™ Express DNA Ladder (#SM1551)
1, 2 – PCR product generated with 2 u of Maxima® Hot Start Taq DNA Polymerase
3, 4 – PCR product generated with Maxima® Hot Start PCR Master Mix (2X) stored at -20°C
5, 6 – PCR product generated with Maxima® Hot Start PCR Master Mix (2X) after 25 freeze-thaw cycles

Patents, Licenses, Trademarks

Protocols & recommendations

RECOMMENDATIONS FOR USE

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