3’-terminal PTO modifications protect from 3’=>5’ exonuclease degradation and ensure efficient priming of DNA synthesis.
Applications
Strand displacement amplification of genomic DNA (2), plasmids and phage DNA (3).
DNA labeling by random priming (4-6).
Description
Exonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions. This primer has two 3'-terminal phosphorothioate (PTO) modifications that are resistant to the 3'=>5' exonuclease activity of proofreading DNA polymerases (1), such as Klenow Fragment and phi29 DNA Polymerase. It also has 5'- and 3'-hydroxyl ends. The Exo-Resistant Random Primer is supplied as a ready-to-use, 20X concentrated aqueous solution.
Quality Control
Functionally tested for the efficient priming of DNA synthesis using phi29 DNA Polymerase.
Notice
Use of this product in certain applications may be covered by patents and may require a license.
Skerra, A., Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity, Nucleic Acids Res., 20, 3551-3554, 1992.
Dean, F.B., et al., Comprehensive human genome amplification using multiple displacement amplification, Proc. Natl. Acad. Sci., 99, 5261-5266, 2002.
Dean, F.B., et al., Rapid amplification of plasmid and phage DNA using phi29 DNA polymerase and multiply-primed rolling circle amplification, Genome Res., 11, 1095-1099, 2001.
Feinberg, A.P., Vogelstein, B., A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity, Anal. Biochem., 132, 6-13, 1983.
Feinberg, A.P., Vogelstein, B., A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity, Addendum, Anal. Biochem., 137, 266-267, 1984.
Mackey, J., et al., Use of random primer extension for concurrent amplification and nonradioactive labeling of nucleic acids, Anal. Biochem., 212, 428-435, 1993.
- Worldwide
- to print
- Store at -20°C
