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Modified Nucleotides

Aminoallyl-dUTP

Product information

Aminoallyl-dTP
Formula: C₁₂H₁₇N₃O₁₄P₃Na₃
Molecular weight: 589.2 (acid form: 523.2)

Applications

• Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random primed labeling or primer extension.

Description

Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH.

Aminoallyl-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, Klenow Fragment, exo- and DNA Polymerase I. The resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten.

General Characteristics

lambda_max = 240 nm, extinction coefficient =11.9x10³ M^-1 x cm^-1 (pH 7.0),
lambda_max = 290 nm, extinction coefficient = 7.8x10³ M^-1 x cm^-1 (pH 7.0).

Quality Control

• Functionally tested in cDNA synthesis with RevertAid™ H Minus Reverse Transcriptase.
• Greater than 95% purity confirmed by HPLC.
• Free of endo-, exodeoxyribonuclease and ribonuclease activities.

Storage

Store at -20°C.

Patents, Licenses, Trademarks

Protocols & recommendations

RECOMMENDATIONS FOR USE