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Kits

GeneJET™ Whole Blood Genomic DNA Purification Mini Kit

Product information

Overview of purification
procedure

Features

• Efficient – high yields of high molecular weight genomic DNA.
• Fast – 20 minute procedure following cell lysis.
• Convenient – spin columns are capped and assembled with collection tubes.
• Pure – DNA pure from enzyme inhibitors, RNA and proteins, ready to use for all demanding applications, validated in real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests.
• Compatible – suitable for wide variety of samples, like fresh or frozen anticoagulated mammalian blood (treated with EDTA, Citrate or Heparine), aged blood, buffy coat, bone marrow, body fluids.

Applications

• Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
  – PCR and qPCR,
  – FastDigest® and conventional restriction digestion,
  – Southern blotting.

Description

The GeneJET™ Whole Blood Genomic DNA Purification Mini Kit is designed for rapid and efficient purification of high quality genomic DNA from whole blood and related body fluids. The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting and enzymatic reactions.

Quality Control

The GeneJET™ Whole Blood Genomic DNA Purification Mini Kit is qualified by isolating genomic DNA from 200 µl of blood following the protocols outlined in the manual. The purified genomic DNA has an A_260/280 ratio between 1.7 and 1.9. A single band of more than 30 kb is observed after agarose gel electrophoresis and ethidium bromide staining. The functional quality of purified genomic DNA is evaluated by PCR amplification of a single-copy gene and by digestion with restriction enzymes.

Components

• Proteinase K Solution
• Lysis Solution
• Wash Buffer WB I (concentrated)
• Wash Buffer II (concentrated)
• Elution Buffer
• GeneJET™ Purification Columns (pre-assembled with collection tubes)
• Collection tubes
• Detailed Protocol

Figure 1. Genomic DNA purified from various sources using the GeneJET™ Whole Blood Genomic DNA Purification Mini Kit.
200 ng of isolated genomic DNA from each source was fractionated by agarose gel (0.5%) electrophoresis.
M – Lambda DNA/HindIII Marker (#SM0101).
1 – human whole blood.
2 – human buffy coat.
3 – human bone marrow.
4 – avian whole blood.

Figure 2. Accurate evaluation of genomic DNA yields.
Genomic DNA was purified from human blood using the GeneJET™ Whole Blood Genomic DNA Purification Mini Kit and kits from other vendors. DNA concentration was estimated using Nanodrop spectrophotometer (Thermo Scientific) and 200 pg used as template in qPCR with primers against single copy gene. Matching yields obtained by qPCR based absolute quantification and spectrophotometrical evaluation indicate absence of reaction inhibitors and contaminating RNA.

Patents, Licenses, Trademarks

Protocols & recommendations

RECOMMENDATIONS FOR USE