Aminoallyl-UTP

- LO certified

Catalog#	Size, concentration	Certificate of Analysis	MSDS

R1091	2.5 µmol (0.05 ml of 50 mM solution)	R1091

Product information

Aminoallyl-UTP
Formula: C₁₂H₁₇N₃O₁₅P₃Na₃
Molecular weight: 605.2 (acid form: 539.2)

Applications

Indirect non-radioactive enzymatic labeling of RNA during in vitro transcription.

Description

Aminoallyl-UTP (5-[3-aminoallyl]-2'-uridine-5'-triphosphate) is supplied as 50 mM aqueous solution titrated to pH 7.0 with NaOH.

Aminoallyl-UTP can be enzymatically incorporated into RNA with T7, T3 and SP6 RNA polymerases. The resulting amine-containing RNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten.

General Characteristics

lambda_max = 240 nm, extinction coefficient = 11.9x10³ M^-1 x cm^-1 (pH 7.0),
lambda_max = 290 nm, extinction coefficient = 7.8x10³ M^-1 x cm^-1 (pH 7.0).

Quality Control

Functionally tested in in vitro transcription with T7 RNA Polymerase.
Greater than 95% purity confirmed by HPLC.
Free of endo-, exodeoxyribonuclease and ribonuclease activities.

Storage

Store at -20°C.

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Protocols & recommendations

RECOMMENDATIONS FOR USE