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Selection Guide

Cloning Workflow	Applications	Products to use
Nucleic Acid Purification	Plasmid DNA	GeneJET™ Plasmid Miniprep Kit
	Genomic DNA	GeneJET™ Genomic DNA Purification Kit
	Total RNA	GeneJET™ RNA Purification Kit
	PCR Products	GeneJET™ PCR Purification Kit
	Gel Extraction	GeneJET™ Gel Extraction Kit
	Removal of RNA from DNA	RNase A, DNase and protease-free
	Removal of DNA from RNA	DNase I, RNase-free
	Removal of proteins from DNA and RNA	Proteinase K (recombinant), PCR grade
	DNA and RNA precipitation	Glycogen, RNA grade
	DNA and RNA precipitation	Glycogen, molecular biology grade
Generation of DNA fragments	Restriction Digestion	FastDigest® Restriction Enzymes
	High Fidelity PCR	Phusion High Fidelity DNA Polymerase
	High Fidelity PCR	Phusion Hot Start II High Fidelity DNA Polymerase
	Hot Start PCR	Phire Hot Start II DNA Polymerase
	PCR	DreamTaq™ DNA Polymerase
		DreamTaq™ Green DNA Polymerase
		DreamTaq™ PCR Master Mix (2X)
		DreamTaq™ Green PCR Master Mix (2X)
		Taq DNA Polymerase (recombinant)
		PCR Master Mix (2X)
	Long PCR	Long PCR Enzyme Mix
	1st stand cDNA synthesis	RevertAid™ Premium First Strand cDNA Synthesis Kit
		RevertAid™ Premium Reverse Transcriptase
		Maxima® Universal First Strand cDNA Synthesis Kit
		Maxima™ Reverse Transcriptase
		RevertAid™ H Minus First Strand cDNA Synthesis Kit
		RevertAid™ H Minus Reverse Transcriptase
		RevertAid™ First Strand cDNA Synthesis Kit
		RevertAid™ Reverse Transcriptase
		RiboLock™ RNase Inhibitor
	2nd stand cDNA synthesis	RNase H
	2nd stand cDNA synthesis	DNA Polymerase I
Electrophoresis	Fast ladders	FastRuler™ High Range DNA Ladder, ready-to-use
		FastRuler™ Low Range DNA Ladder, ready-to-use
		FastRuler™ Middle Range DNA Ladder, ready-to-use
		Ultra Low Range DNA Ladder, ready-to-use
	1 kb ladders	GeneRuler™ 1 kb DNA Ladder
		GeneRuler™ 1 kb DNA Ladder, ready-to-use
		O.GeneRuler™ 1 kb DNA Ladder, ready-to-use
		GeneRuler™ 1 kb Plus DNA Ladder
		GeneRuler™ 1 kb Plus DNA Ladder, ready-to-use
		O.GeneRuler™ 1 kb Plus DNA Ladder, ready-to-use
	100 bp ladders	GeneRuler™ 100 bp DNA Ladder
		GeneRuler™ 100 bp DNA Ladder, ready-to-use
		O.GeneRuler™ 100 bp DNA Ladder, ready-to-use
		GeneRuler™ 100 bp Plus DNA Ladder
		GeneRuler™ 100 bp Plus DNA Ladder, ready-to-use
		O.GeneRuler™ 100 bp Plus DNA Ladder, ready-to-use
	50 bp ladders	GeneRuler™ 50 bp DNA Ladder
		GeneRuler™ 50 bp DNA Ladder, ready-to-use
		O.GeneRuler™ 50 bp DNA Ladder, ready-to-use
	High range ladders	GeneRuler™ High Range DNA Ladder
	High range ladders	GeneRuler™ High Range DNA Ladder, ready-to-use
	RNA ladders	RiboRuler™ High Range RNA Ladder
		RiboRuler™ High Range RNA Ladder, ready-to-use
		RiboRuler™ Low Range RNA Ladder
		RiboRuler™ Low Range RNA Ladder, ready-to-use
	Loading Dyes	6X DNA Loading Dye
		6X Orange DNA Loading Dye
		2X RNA Loading Dye
	Agarose	TopVision™ Agarose
	Agarose	TopVision™ Low Melting Point Agarose
	Electrophoresis buffers	50X TAE Buffer (Tris-acetate-EDTA)
	Electrophoresis buffers	10X TBE Buffer (Tris-borate-EDTA)
Modification of DNA Ends	Blunting of DNA fragment	Fast DNA End Repair Kit
		T4 DNA Polymerase
		Klenow Fragment
		S1 Nuclease
	Dephosphorylation	FastAP™ Thermosensitive Alkaline Phosphatase
	Phosphorylation of PCR products or synthetic DNA	T4 Polynucleotide Kinase
Ligation	Ligation	CloneJET™ PCR Cloning Kit
		Rapid DNA Ligation Kit
		T4 DNA Ligase
Transformation		TransformAid™ Bacterial Transformation Kit

Additional Products

Applications	Procedures	Products to use
Cloning vectors	closed circular plasmids	pUC18 DNA
Cloning vectors	closed circular plasmids	pUC19 DNA
Cloning vectors	closed circular plasmids	pUC57 DNA
Cloning vectors	closed circular plasmids	pTZ19R DNA
Cloning vectors	closed circular plasmids	pBR322 DNA
Cloning vectors	linearized and dephosphorylated vectors	pUC19 DNA/BamHI, Digested & Dephosphorylated
Cloning vectors	linearized and dephosphorylated vectors	pUC19 DNA/SmaI, Digested & Dephosphorylated
Reaction set up	nucleotides	dNTP Mix, 2 mM each
Reaction set up	nucleotides	dNTP Mix, 10 mM each
Reaction set up	nucleotides	dNTP Mix, 25 mM each
Reaction set up	nucleotides	dNTP Set
Reaction set up	water	Water, nuclease-free
Reaction set up	water	DEPC-treated Water
Reaction set up	primers	M13/pUC sequencing primer (-20), 17-mer 5'-d(GTAAAACGACGGCCAGT)-3'
Reaction set up	primers	SP6 promoter sequencing primer, 18-mer 5'-d(ATTTAGGTGACACTATAG)-3'
Reaction set up	primers	Oligo(dT)₁₈ Primer
Reaction set up	primers	Random Hexamer Primer
Reaction set up	primers	Exo-Resistant Random Primer
Cultivation of E.coli	microbiological media	FastMedia™ LB Liquid Amp
Cultivation of E.coli	media additives	X-Gal
Cultivation of E.coli	media additives	X-Gal Solution, ready-to-use
Cultivation of E.coli	media additives	IPTG, dioxane-free
Cultivation of E.coli	media additives	IPTG Solution, ready-to-use
Transfection	transfection reagents	TurboFect™ Transfection Reagent
Transfection	transfection reagents	ExGen 500 in vitro Transfection Reagent
Transfection	transfection reagents	ExGen 500 in vivo Transfection Reagent
Transfection	transfection reagents
Transfection	transfection reagents	TurboFect™ in vivo Transfection Reagent