Use these tools to plan your experiments:
- REsearch™ – find restriction enzymes either by name or recognition sequence
- DoubleDigest™ – perform double digestion with Fermentas restriction enzymes
- REviewer™ – use for DNA sequence analysis, plasmid map creation and biochemical calculations
- to print
Conventional Restriction Enzymes
10X Buffer Eco52I
| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| B22 | 1 ml | B22 |
- Product information
-
DescriptionThe following enzymes require unique buffers for optimal digestion: AarI, AjiI, BamHI, BfuI, Bpu10I, BseXI, Bsp143I, Cfr9I, Cfr10I, Eam1105I, Ecl136II, Eco52I, EcoRI, KpnI, Lsp1109I, PacI, PasI, Ppu21I, SacI, ScaI, SdaI, SduI, SgeI and TaqI.
To ensure consistent enzyme performance, Fermentas restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.
Fermentas restriction enzymes exhibit 100% of their certified activity in the recommended buffer.
1X Composition- 10 mM Tris-HCl (pH 8.5 at 37°C)
- 3 mM MgCl2
- 100 mM NaCl
- 0.1 mg/ml BSA
StorageAll Fermentas restriction enzyme buffers should be stored at -20°C.
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