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TurboFect™ in vitro Transfection Reagent
The highest transfection efficiency at the best price

#R0531	1 ml (delivers 500 µg of DNA)
Related Documents (in pdf, ~30-50 KB): Certificate of Analysis: #R0531 MSDS (English) MSDS (English-USA) MSDS (German) Flyer  (in pdf, 162 KB)

 

Features

• High transfection efficiency of a wide variety of cell types:
  • primary and secondary cell lines,
  • adherent and suspension cells,
  • differentiated and undifferentiated cells,
  • stable and transient transfections.
• No optimization required for most cell lines.
• 1 ml delivers 500 µg of DNA.
• Excellent transfection efficiency in the presence or absence of serum.
• Minimal cytotoxicity.
• Ready-to-use reagent - no need to reconstitute, dilute or manipulate.
• Simple protocol.

Description
TurboFect™ in vitro Transfection Reagent is a sterile solution of a proprietary cationic polymer in water. The polymer forms compact, stable, positively charged complexes with DNA. These complexes protect DNA from degradation and facilitate gene delivery into eukaryotic cells (see Fig.1). TurboFect™ is ideal for transfection of a variety of cells, including primary and difficult-to-transfect cells (see Fig.1 and list of cells transfected with TurboFect™). Transfection can be performed in the presence or absence of serum. TurboFect™ demonstrates superior transfection efficiency and minimal toxicity when compared to lipid-based or other polymer-based transfection reagents (see Fig.2).

Storage
Store at 4°C.

Cells Successfully Transfected with TurboFect™

• Permanently growing cell lines:
  • B50 rat nervous tissue neuronal cells
  • BHK 21 baby hamster kidney cells
  • Calu1 human lung epidermoid carcinoma cells
  • CHO chinese hamster ovary cells
  • COLO human colon adenocarcinoma cells
  • Cos-7 african green monkey kidney cells
  • HEK293 human embryonic kidney cells
  • HeLa human cervix adenocarcinoma cells
  • HeLa S3 human cervix carcinoma cells
  • Hep2C human larynx carcinoma cells
  • HepG2 human hepatoma cells
  • Jurkat human leukaemic T cells
  • L929 mouse connective tissue fibroblasts
  • MDCK Madin Darby Canine Kidney cells
  • NIH3T3 mouse embryo fibroblasts
  • RAW264 mouse leukaemic monocyte-macro phage cells
  • Sp2/Ag14 mouse myeloma cells
  • WEHI mouse B cell lymphoma cells
• Primary cell cultures:
  • HLF human lung fibroblasts
  • Mouse bone marrow derived dendritic cells
  • Mouse bone marrow derived macrophages
  • Rat fibroblasts

Protocol for in vitro Transfection with TurboFect™ Transfection Reagent in a 24-well Plate

Figure 1. EGFP expression in cell lines transfected with TurboFect™ in vitro Transfection Reagent.
A - primary mouse dendritic cells derived from bone marrow, B - HeLa cells

Figure 2. Comparison of Fermentas TurboFect™ in vitro transfection reagent with tranfection reagents from other vendors.
HeLa cells were transfected with plasmid DNA encoding eGFP. Transfections were performed according to manufacturers recommendations and analyzed by flow cytometry.

Updated balandžio 02, 2008 08:47