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ExGen 500 in vivo Transfection Reagent

#R0521 0.1 ml
(for 20-30 in vivo transfections)

Related Documents (in pdf, ~30-50 KB):

Certificate of Analysis: #R0521
MSDS (English)
MSDS (English-USA)
MSDS (German)

Description
 ExGen 500 is a sterile solution of linear polyethylenimine (PEI) molecules (22 kDa) in water at a concentration of 100 mM in terms of nitrogen residues. PEI has a high cationic-charge density potential which makes it an excellent DNA condensing and gene-delivering agent (1, 2).
In a glucose solution, ExGen 500 in vivo Transfection Reagent and DNA form cationic microprecipitates with a diameter of approximately 50 nm as a result of charge interaction. The microprecipitates attach to cellular proteoglycans and then penetrate the cell by endocytosis (1).
Every third atom of the ExGen 500 backbone is a protonable amino nitrogen atom, which makes the polymeric association an effective “proton sponge” (1, 3). This property allows for endosome buffering and protects DNA from lysosomal degradation.
ExGen 500 in vivo Transfection Reagent permits in vivo transfection of DNA, regardless of the size.

Concentration 
The solution is 100 mM in terms of nitrogen residues.

Reagent not Supplied
Sterile 5% w/v glucose solution for dilution of DNA.

DNA Quality Requirements
The A260/A280 ratio should be at least 1.8. It is recommended to have DNA preparation containing the minimal amount of contaminant endotoxins (<0.1 EU/1µg DNA).

Transfection in vivo

Considerations for in vivo gene delivery with ExGen 500

References

  1.  Godbey, W.T., Wu, K.K., Mikos, A.G., Poly(ethylenimine) and its role in gene delivery, J. Controlled Release, 60, 149-160, 1999.

  2. Goula, D., et al., Size, diffusibility and transfection performance of linear PEI/DNA complexes in the mouse central nervous system, Gene Therapy, 5, 712-717, 1998.
  3. Behr, J.P., L'eponge a protons: un moyen d'entrer dans une cellule auquel les virus n'ont pas pense, Medicine/Sciences, 12, 56-59, 1996.
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Updated gegužės 29, 2008 10:22