NBT
#R0841 1.0g #R0842 5.0g Formula: C40H30N10O6Cl2 Molecular Weight: 817.7 Related Documents (in pdf, ~30-50 KB):
Certificate of Analysis: #R0841, #R0842
MSDS (English)
MSDS (English-USA)
MSDS (German)Description
NBT (nitro blue tetrazolium) is an oxidant, used in combination with BCIP, or BCIP derivatives (BCIP-T) (see protocol below), to enhance the development of blue color during detection of alkaline phosphatase activity. It is suitable for immunohistochemistry and cytochemistry; NBT is also a substrate for oxidases and dehydrogenases.Applications
- When alkaline phosphatase conjugates are used for detection, NBT is used for the following applications: Southern blotting, Western blotting, Northern blotting and dot/slot blots.
- The NBT is highly recommended for use in a conjunction with the Fermentas Biotin DecaLabel™ DNA Labeling Kit.
- The BCIP-T/NBT solution is included in the Fermentas Biotin Chromogenic Detection Kit.
Quality Control
One spot by TLC. Less than 8% water.Note
Preparation of a 75 mg/ml stock solution in 70% dimethylformamide is recommended.Storage
Store at +4°C in the dark.Protocol for Preparation of BCIP-T/NBT Substrate Solution
- Add the following components to 10 ml of the Alkaline Phosphatase (AP) buffer (100 mM Tris-HCl (pH 9.5), 100 mM NaCl and 10 mM MgCl2):
BCIP-T (50 mg/ml in dimethylformamide) 33 µl NBT (75 mg/ml in 70%dimethylformamide) 44 µl Note
- Avoid exposure to light.
- Prepare fresh developing solution and use within an hour.
- BCIP-T and NBT solutions in dimethylformamide do not freeze. They are stable for approximately 2 years when stored at -20°C in the dark.
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Updated vasario 27, 2008 12:38
