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IPTG, dioxane-free

#R0391 1.0g
#R0392 5.0g
#R0393 25.0g
Formula: C9H18O5S
Molecular Weight: 238.3

Related Documents (in pdf, ~30-50 KB):

Certificate of Analysis: #R0391, #R0392, #R0393
MSDS (English)
MSDS (English-USA)
MSDS (German)

Description
IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. The IPTG is used to induce the expression of cloned genes which are under control of the lac operon (1). It is used in conjunction with X-Gal to determine the lac phenotype in blue/white colony screening.

Applications

Quality Control
Dioxane-free.
Greater than 99% purity confirmed by HPLC.
Functionally tested in blue/white colony screening.

Note

Storage
Store at -20°C.

Protocol for the Blue/White Colony Screening

For individual LB (Luria Broth) agar plates:

  1. Pour sterile warm LB agar (about 25 ml) into a Petri dish.
  2. Dry opened LB plates at room temperature under UV light for about 30 min.
  3. Add 40 µl of the X-Gal Solution (20 mg/ml), ready-to-use.
  4. Add 40 µl of 100 mM IPTG Solution, ready-to-use.
  5. Spread evenly on the plate with a sterile spatula.

For batch use, add the following directly per 1 ml of the liquid LB agar (kept at about 50°C):

  1. 1 µl of X-Gal Solution (20 mg/ml), ready-to-use.
  2. 1 µl of 100 mM IPTG Solution, ready-to-use.
  3. Mix well.
  4. Pour 25 ml of prepared LB agar into each Petri dish.
  5. Dry opened LB plates at room temperature under UV light for about 30 min.

Reference

  1. Sambrook, J., Russell, D.W., Molecular Cloning: A Laboratory Manual, the Third edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1.124-1.125, A1.27, 2001.

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Updated vasario 27, 2008 12:35