0.5 M EDTA, pH 8.0, molecular biology grade
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Certificate of Analysis: #R1021
MSDS (English)
MSDS (English-USA)
MSDS (German)Description
0.5 M EDTA, pH 8.0, is 0.22 µm membrane-filtered aqueous solution of ethylendiaminetetraacetic acid (EDTA), sodium salt. It is suitable for biochemistry or molecular biology applications that require a chelator for divalent cations. By forming complexes with metal ions, EDTA inhibits metal-dependent enzymatic reactions. The EDTA complexes metal ions at a 1:1 ratio.
Nucleic acids are usually stored in TE buffer that contains 1 mM EDTA.Quality Control
The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests.Storage
Store at +4°C.
Usage Recommendations For immediate inhibition of metal-dependent enzymatic reactions, add 1 µl of 0.5 M EDTA to 20 µl reaction mixture. Mix well. Note
- For visualization of the reaction products on an agarose gel:
- mix 5 volumes of the reaction mixture with 1 volume of the 6X DNA Loading Dye;
- load the mixture on a gel and run electrophoresis.
- For usage of the reaction products in further enzymatic reactions:
- after the addition of EDTA, the reaction mixture should be purified using Fermentas DNA Gel Extraction Kit or by phenol/chloroform extraction and alcohol precipitation (see protocol).
- For storage of reaction mixtures:
- after the addition of EDTA, all enzymes in the reaction mixture should be thermally inactivated if possible.
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Updated vasario 06, 2008 16:19
