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DTT, molecular biology grade

#R0861	5.0g
#R0862	25.0g
Formula:	C4H10O2S2
Molecular Weight:	154.25
Related Documents (in pdf, ~30-50 KB): Certificate of Analysis: #R0861, #R0862 MSDS (English) MSDS (English-USA) MSDS (German)

Description
DTT (dithiothreitol; Cleland’s reagent) is used to quantitatively reduce disulfide bonds and maintain monothiols in a reduced state (1). At low concentrations, DTT stabilizes enzymes and other proteins which possess free sulfhydryl groups and has been shown to restore activity lost by oxidation of these groups in vitro.
At a final 0.1 M concentration DTT is also widely used for disruption of protein disulfide bonds in SDS-polyacrylamide gel electrophoresis.

Quality Control
The absence of endodeoxyribonucleases, exodeoxyribonucleases, ribonucleases and phosphatases is confirmed by appropriate quality tests.
Greater than 99% of titratable SH groups is confirmed by iodometric method.

Note
DTT is not stable in solution. Only freshly-made DTT solutions should be used.

Storage
Store at +4°C.

Reference

1. Cleland, W.W., Dithiothreitol, a new protective reagent for SH groups, Biochemistry, 3, 480-2, 1964.

Updated vasario 27, 2008 12:35