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BCIP-T

#R0821	1.0g
#R0822	5.0g
Formula:	C8H6NO4 BrClP x C7H9N
Molecular Weight:	433.6
Related Documents (in pdf, ~30-50 KB): Certificate of Analysis: #R0821, #R0822 MSDS (English) MSDS (English-USA) MSDS (German)

Description
BCIP-T (5-bromo-4-chloro-3-indolyl phosphate, p-toluidine salt) is a chromogenic substrate for alkaline phosphatase, used in combination with the oxidant NBT (nitro blue tetrazolium) to enhance blue color development.

Applications

• When alkaline phosphatase conjugates are used for detection, BCIP-T is recommended for the following applications: Southern blotting, Western blotting, Northern blotting and dot/slot blots.
• The BCIP-T is highly recommended for use in a conjunction with the Fermentas Biotin DecaLabel™ DNA Labeling Kit.
• The BCIP-T/NBT solution is included in the Fermentas Biotin Chromogenic Detection Kit.

Quality Control
Less than 3% moisture content.

Note
Preparation of a 50 mg/ml stock solution in dimethylformamide is recommended.

Storage
Store at -20°C in the dark.

Protocol for Preparation of BCIP-T/NBT Substrate Solution

1. Add the following components to 10 ml of the Alkaline Phosphatase (AP) buffer (100 mM Tris-HCl (pH 9.5), 100 mM NaCl and 10 mM MgCl₂):

   BCIP-T (50 mg/ml in dimethylformamide)	33 µl
   NBT (75 mg/ml in 70%dimethylformamide)	44 µl

Note

• Avoid exposure to light.
• Prepare fresh developing solution and use within an hour.
• BCIP-T and NBT solutions in dimethylformamide do not freeze. They are stable for approximately 2 years when stored at -20°C in the dark.

Related Products

NBT Biotin DecaLabel™ DNA Labeling Kit Biotin Chromogenic Detection Kit

Updated vasario 27, 2008 12:38