Home  Contacts  Order  Catalog  Support  Search  Alphabetical Index  Numerical Index  Restriction Enzymes  Modifying Enzymes  PCR, qPCR, RT-PCR & dNTPs  Molecular Cloning  Nucleic Acid Purification  Molecular Labeling & Detection  In vitro Transcription  Electrophoresis Products  Nucleotides  Transfection Reagents  Reagents C A T A L O G

TscAI (TspRI)
Available in FastDigest® format

C 5'- N N C A G T G N N^-3' 3'-^N N G T C A C N N -5' G		digested lambda DNA 1.4% agarose
#ER2101 Supplied with: 10X Buffer Tango™	1000 u   1 ml
Commercial Isoschizomers: TspRI
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER2101 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer Tango™	65°C	0.2	No	50-100	50-100	20-50	20-50	100	20-50

Storage Buffer
TscAI is supplied in: 10 mM Tris-HCl (pH 7.4 at 25°C), 200 mM NaCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.

Ligation and Recleavage
After 50-fold overdigestion with TscAI, more than 95% of the DNA fragments can be ligated and recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  never overlaps  never overlaps  may overlap  may overlap	- no effect. - no effect. - no effect. - effect not determined. - effect not determined.

Note

• Incubation at 37°C results in less than 5% activity.
• Low salt, high glycerol (>5%) concentrations, pH >8.0 or a large excess of enzyme may result in star activity.
• TscAI produces DNA fragments with a 9-base 3'-extension. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye & SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer Tango™	100	20-50

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
119	6	8	12	10	10	10	10	10	15	13

Updated balandžio 22, 2008 11:36