Home  Contacts  Order  Catalog  Support  Search  Alphabetical Index  Numerical Index  Restriction Enzymes  Modifying Enzymes  PCR, qPCR, RT-PCR & dNTPs  Molecular Cloning  Nucleic Acid Purification  Molecular Labeling & Detection  In vitro Transcription  Electrophoresis Products  Nucleotides  Transfection Reagents  Reagents C A T A L O G

Tru1I (MseI)
Available in FastDigest® format

5'-T^T A A-3' 3'-A A T^T-5'		digested lambda DNA 1.4% agarose
#ER0981 Supplied with: 10X Buffer R 10X Buffer Tango™	300 u   1 ml 1 ml
#ER0982 Supplied with: 10X Buffer R 10X Buffer Tango™	1500 u   1 ml 1 ml
#ER0983 Supplied with: 10X Buffer R 10X Buffer Tango™	HC, 1500 u   1 ml 1 ml
Commercial Isoschizomers: MseI, Tru9I
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER0981, #ER0982, #ER0983 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl; 50 u/µl, HC

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer R	65°C	0.2	No	50-100	50-100	20-50	100	50-100	100

Storage Buffer
10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with Tru1I, more than 90% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  never overlaps  never overlaps  may overlap  never overlaps	- no effect. - no effect. - no effect. - blocked. - no effect.

Compatible Ends
AseI, BfaI, Csp6I, FspBI, NdeI, VspI.

Note
Incubation at 37°C results in 10% activity. We recommend using Tru1I, HC (#ER0983) at 37°C.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer R	50-100	100

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
195	35	63	15	13	13	18	19	19	16	12

Updated balandžio 22, 2008 11:36