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C A T A L O G
 

TauI

       G
5'-G C C G^C-3'
3'-C^G G C G-5'
       C

digested lambda DNA
1.4% agarose
      
#ER1651
 Supplied with:
10X Buffer B
10X Buffer Tango™		 50 u
 
1 ml
1 ml
#ER1652
 Supplied with:
10X Buffer B
10X Buffer Tango™		 250 u
 
1 ml
1 ml
Commercial Isoschizomers: -

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER1651, #ER1652
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
2 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 2X
Buffer B 55°C 1.0 No 100 50-100 0-20 0-20 20-50 0-20

Storage Buffer
 10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 10-fold overdigestion with TauI, more than 90% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 never overlaps
 completely overlaps
 never overlaps
 never overlaps		 - no effect.
- no effect.
- blocked.
- no effect.
- no effect.

Note
  • Incubation at 37°C results in 30% activity.
  • TauI may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid an atypical DNA band pattern, use the 6X Loading Dye & SDS Solution (#R1151) for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer B 20-50 NR

NR: buffer is not recommended, since enzyme activity is less than 20%.

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
181 17 7 21 7 7 8 10 10 12 22

See also General Properties of Restriction Enzymes:

 

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Updated kovo 07, 2008 15:29