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C A T A L O G
 

TatI

5'-(A/T)^G T A C (A/T)-3'
3'-(A/T) C A T G^(A/T)-5'

digested lambda DNA
1.0% agarose
     
#ER1291
Supplied with:
10X Buffer Tango™		 100 u
 
1 ml
#ER1292
Supplied with:
10X Buffer Tango™		 500 u
 
1 ml
Commercial Isoschizomers: -

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER1291, #ER1292
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
5 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 2X
Buffer Tango™ 65°C
under paraffin oil in a capped vial		 0.2 No NR 50-100* 20-50 20-50 100* 0-20

NR: buffer is not recommended, because of high star activity.
* Star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).

Storage Buffer
 10 mM Tris-HCl (pH 7.5 at 25°C), 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Reclevage
After 5-fold overdigestion with TatI, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 never overlaps
 never overlaps
 never overlaps
 may overlap		 - no effect.
- no effect.
- no effect.
- no effect.
- effect not determined.

Note
  • Incubation at 37°C results in 20% activity.
  • Greater than 5-fold overdigestion with TatI may result in star activity.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer Tango™ 100* NR

* Star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).
NR: buffer is not recommended, since enzyme activity is less than 20%.

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
24 0 5 2 2 2 1 1 1 1 1

See also General Properties of Restriction Enzymes:

 

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Updated kovo 07, 2008 15:28