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TasI (Tsp509I)
 Available in FastDigest® format

5'-^A A T T -3'
3'- T T A A^-5'

digested lambda DNA
1% agarose
     
#ER1351
Supplied with:
10X Buffer B
10X Buffer Tango™		 1000 u
 
1 ml
1 ml
#ER1352
Supplied with:
10X Buffer B
10X Buffer Tango™		 5000 u
 
2x1 ml
1 ml
Commercial Isoschizomers:
Sse9I, Tsp509I, TspEI

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER1351, #ER1352
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 2X
Buffer B 65°C
under paraffin oil in a capped vial		 0.3 No 100 50-100 20-50 0-20 20-50 0-20

Storage Buffer
 10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with TasI, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 never overlaps
 never overlaps
 never overlaps
 may overlap		 - no effect.
- no effect.
- no effect.
- no effect.
- blocked.

Note
Incubation at 37°C results in less than 10% activity

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer B 20-50 NR

NR: buffer is not recommended, since enzyme activity is less than 20%.

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
189 25 64 8 7 7 10 12 12 14 13

See also General Properties of Restriction Enzymes:

 

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Updated balandžio 22, 2008 11:36