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TaqI
Available in FastDigest® format

5'-T^C G A-3' 3'-A G C^T-5'		digested lambda DNA (dam-) 1.4% agarose
#ER0671 Supplied with: 10X Buffer TaqI 10X Buffer Tango™	3000 u   2x1 ml 1 ml
#ER0672 Supplied with: 10X Buffer TaqI 10X Buffer Tango™	5x3000 u   4x1 ml 1 ml
#ER0673 Supplied with: 10X Buffer TaqI 10X Buffer Tango™	HC, 15000 u   4x1 ml 1 ml
Commercial Isoschizomers: -
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER0671, #ER0672, #ER0673 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl,
50 u/µl, HC

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				TaqI (unique)	B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	1X	2X
Buffer TaqI	65°C  under paraffin oil in a capped vial	0.3	No	100	0-20	20-50	20-50	20-50	20-50	20-50

Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C), 300 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with TaqI, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	may overlap  never overlaps  completely overlaps  never overlaps  never overlaps	- blocked. - no effect. - no effect. - no effect. - no effect.

Compatible Ends
AceI, AciI, AclI, BsaHI, Bsp119I, Bsu15I, ClaI, Hin1I, Hin6I, HinP1I, HpaII, MaeII, MspI, NarI, Psp1406I, SsiI, XmiI.

Note

Assayed using lambda DNA (dam-) (#SD0021). Incubation at 37°C results in 10% activity. We recommend using TaqI, HC (#ER0673) at 37°C.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer TaqI	20-50	20-50

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
121	10	12	7	4	4	5	7	7	10	10

Updated balandžio 22, 2008 11:36