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TaiI (MaeII*)
Available in FastDigest® format

* Unlike MaeII, TaiI produces fragments with a 4-base 3'-extension		digested lambda DNA 1.4% agarose
5'- A C G T^-3' 3'-^T G C A -5'
#ER1141 Supplied with: 10X Buffer R 10X Buffer Tango™	400 u   1 ml 1 ml
#ER1142 Supplied with: 10X Buffer R 10X Buffer Tango™	2000 u   1 ml 1 ml
Commercial Isoschizomers: (HpyCH4IVm), (MaeII) "m" indicates different sensitivity to methylation. Enzymes in parentheses have different cleavage specificities.
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER1141, #ER1142 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer R	65°C	0.3	No	50-100	50-100	20-50	100	100	50-100

Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with TaiI, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  never overlaps  completely overlaps  may overlap  may overlap	- no effect. - no effect. - blocked. - effect not determined. - effect not determined.

Compatible Ends
AatII.

Note
Incubation at 37°C results in less than 10% activity.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer R	100	50-100

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
143	19	22	10	5	5	8	8	8	7	9

Updated balandžio 22, 2008 11:36