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TaaI (HpyCH4III)
Available in FastDigest® format

5'-A C N^G T-3'
3'-T G^N C A-5'

digested lambda DNA
1.4% agarose
      
#ER1361
Supplied with:
10X Buffer Tango™		 200 u
 
1 ml
#ER1362
Supplied with:
10X Buffer Tango™		 1000 u
 
1 ml
Commercial Isoschizomers:
Bst4CI, HpyCH4III

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER1361, #ER1362
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 2X
Buffer Tango™ 65°C 0.2 No 0-20 0-20 0-20 50-100 100 100

Storage Buffer
 10 mM Tris-HCl (pH 7.5 at 25°C), 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with TaaI, approximately 90% of the digested DNA fragments can be ligated and more than 90% of these can be recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 never overlaps
 may overlap
 may overlap
 may overlap		 - no effect.
- no effect.
- cleavage impaired.
- effect not determined.
- effect not determined.

Compatible Ends
Hpy188I.

Note
Incubation at 37°C results in 10% activity.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer Tango™ 100 100

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
187 15 31 14 8 8 7 8 8 14 20

See also General Properties of Restriction Enzymes:

 

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Updated balandžio 22, 2008 11:36