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SsiI (AciI)
Available in FastDigest® format

5'-C^C G C-3' 3'-G G C^G-5'		digested lambda DNA 2.0% agarose
#ER1791 Supplied with: 10X Buffer O 10X Buffer Tango™	200 u   1 ml 1 ml
Commercial Isoschizomers: AciI, BspACI
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER1791 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer O	37°C	0.5	65°C	NR	20-50	100	50-100	NR	100

NR: buffer is not recommended, because of high star activity.

Storage Buffer
10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recutting
After 50-fold overdigestion with SsiI, approximately 95% of the digested DNA fragments can be ligated. No more than 50% of these can be recut due to asymmetric recognition sequence of SsiI. The remaining uncleaved ligation products may be cut by HhaI or HpaII.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  never overlaps  completely overlaps  never overlaps  never overlaps	- no effect. - no effect. - blocked. - no effect. - no effect.

Compatible Ends
AccI (GT/CGAC), AclI, BsaHI (GR/CGCC), Bsp119I, Bsu15I, ClaI, Hin1I (GR/CGCC), Hin6I, HinP1I, HpaII, MspI, NarI, Psp1406I, TaqI, XmiI (GT/CGAC).

Note
Low salt, high glycerol (>5%) concentrations, pH >8.0 or large excess of the enzyme may result in star activity.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer O	NR	100

NR: buffer is not recommended, because of high star activity.

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
516	36	42	67	34	34	32	36	36	32	56

Updated balandžio 22, 2008 11:37