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SmuI (FauI)

5'-C C C G C (N)4-3' 3'-G G G C G (N)6-5'		digested lambda DNA 1.0% agarose
#ER1691 Supplied with: 10X Buffer Tango™	50 u   1 ml
#ER1692 Supplied with: 10X Buffer Tango™	250 u   1 ml
Commercial Isoschizomers: FauI
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER1691, #ER1692 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
1 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer Tango™	37°C	0.2	65°C	50-100	50-100	0-20	20-50	100	20-50

Storage Buffer
10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 10-fold overdigestion with SmuI, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  never overlaps  completely overlaps  never overlaps  never overlaps	- no effect. - no effect. - blocked. - no effect. - no effect.

Note
Greater than 40-fold overdigestion with SmuI may result in star activity.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer Tango™	100	20-50

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
90	0	10	10	5	5	5	5	5	1	10

Updated balandžio 22, 2008 11:37