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SmiI (SwaI)
Available in FastDigest® format

5'-A T T T^A A A T-3' 3'-T A A A^T T T A-5'		digested Ad2 DNA 0.7% agarose
#ER1241 Supplied with: 10X Buffer O 10X Buffer Tango™	1000 u   1 ml 1 ml
#ER1242 Supplied with: 10X Buffer O 10X Buffer Tango™	5000 u   2x1 ml 1 ml
Commercial Isoschizomers: SwaI
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER1241, #ER1242 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer O	30°C	0.1	65°C	0-20	0-20	100	20-50	0-20	20-50

Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C), 200 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with SmiI, more than 80% of the digested DNA fragments can be ligated in a reaction mixture containing 20-40 u of T4 DNA Ligase/1 µg of fragments and 10% PEG. More than 95% of these can be recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  never overlaps  never overlaps  never overlaps  may overlap	- no effect. - no effect. - no effect. - no effect. - effect not determined.

Digestion of Agarose-embedded DNA
Minimum 10 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded Adenovirus-2 DNA in 16 hours.

Note

Assayed using Adenovirus-2 DNA. Incubation at 37°C results in 70% activity.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer O	NR	20-50

NR: buffer is not recommended, since enzyme activity is less than 20%.

Number of Recognition Sites in DNA Molecules

Ad2	Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
1	0	0	1	0	0	0	0	0	0	0	0

Updated balandžio 22, 2008 11:37