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SmaI
Available in FastDigest® format

5'-C C C^G G G-3' 3'-G G G^C C C-5'		digested lambda DNA 0.7% agarose
#ER0661 Supplied with: 10X Buffer Tango™	1200 u   1 ml
#ER0662 Supplied with: 10X Buffer Tango™	5x1200 u   2x1 ml
#ER0663 Supplied with: 10X Buffer Tango™	HC, 6000 u   2x1 ml
#ER0665 Supplied with: 10X Buffer Tango™	2000 u   1 ml
Commercial Isoschizomers: (Cfr9Im), (TspMIm), (XmaCI), (XmaIm) Enzymes in parentheses have different cleavage specificities. "m" indicates different sensitivity to methylation.
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER0661, #ER0662, #ER0663, #ER0665 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl; 50 u/µl, HC

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer Tango™	30°C	0.2	65°C	50-100	0-20	0-20	0-20	100	0-20

Storage Buffer
10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with SmaI, more than 90% of the digested pUC19 DNA fragments can be ligated and recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  never overlaps  completely overlaps  never overlaps  never overlaps	- no effect. - no effect. - blocked. - no effect. - no effect.

Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Note

• Incubation at 37°C results in 50% activity.
• Incubation at 25°C results in 100% activity.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer Tango™	100	NR

NR: buffer is not recommended, since enzyme activity is less than 20%.

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
3	0	1	0	1	1	1	1	1	1	0

Updated balandžio 22, 2008 11:37