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SgrDI

5'-C G^T C G A C G-3' 3'-G C A G C T^G C-5'		digested lambda DNA 1.0% agarose
#ER2031 Supplied with: 10X Buffer R 10X Buffer Tango™	200 u   1 ml 1 ml
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER2031 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
5 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer R	37°C	0.3	65°C	0-20	0-20	0-20	100	NR	100

NR: buffer is not recommended, because of high star activity.

Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C), 300 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 30-fold overdigestion with SgrDI, more than 80% of the digested DNA fragments can be ligated. More than 95% of these sites can be recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	may overlap  never overlaps  completely overlaps  never overlaps  never overlaps	- no effect. - no effect. - blocked. - no effect. - no effect.

Compatible Ends
SalI, Eco88I, SmiI, XhoI.

Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Note
Low salt concentration or large excess of the enzyme may result in star activity.

Double Digestions using Tango™ Buffer

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer O	NR	100

NR: buffer is not recommended, because of high star activity.

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
1	0	0	0	0	0	0	0	0	0	0

Updated rugsėjo 03, 2008 12:35