Home  Contacts  Order  Catalog  Support
 Search  Alphabetical Index  Numerical Index
 Restriction Enzymes  Modifying Enzymes  PCR, qPCR, RT-PCR & dNTPs  Molecular Cloning  Nucleic Acid Purification
 Molecular Labeling & Detection  In vitro Transcription  Electrophoresis Products  Nucleotides  Transfection Reagents  Reagents
C A T A L O G
 

SduI (Bsp1286I)
 Available in FastDigest® format

     T     T
     A     A
5'-G G G C C^C-3'
3'-C^C C G G G-5'
     T     T
     A     A

digested lambda DNA
1.0% agarose
     
#ER0651
 Supplied with:
10X Buffer SduI		 500 u
 
1 ml
Commercial Isoschizomers:
Bsp1286I, MhlI

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER0651
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
SduI
(unique)		 B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 1X 2X
Buffer SduI 37°C 0.3 65°C 100 NR 50-100* 50-100 0-20 NR NR

* Star activity appears at 5-fold overdigestion (5 units x 1 hour).
NR: buffer is not recommended, because of high star activity.

Storage Buffer
 10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM EDTA, 1 mM DTT, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recutting
After 50-fold overdigestion with SduI, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 may overlap
 may overlap
 may overlap
 may overlap		 - no effect.
- no effect.
- no effect.
- effect not determined.
- effect not determined.

Note
Low salt, high glycerol (>5%) concentrations, pH >8.0 or large excess of the enzyme may result in star activity.

Double Digestions using Tango™ Buffer

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer SduI NR NR

NR: buffer is not recommended, because of high star activity.

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
38 3 5 10 5 6 5 6 6 4 8

See also General Properties of Restriction Enzymes:

 

 Home  Search  Contacts  Order  Catalog  Support

Updated balandžio 22, 2008 11:33