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SdaI (SbfI)
Available in FastDigest® format

5'-C C T G C A^G G-3' 3'-G G^A C G T C C-5'		digested lambda DNA 0.7% agarose
#ER1191 Supplied with: 10X Buffer SdaI 10X Buffer Tango™	300 u   1 ml 1 ml
#ER1192 Supplied with: 10X Buffer SdaI 10X Buffer Tango™	1500 u   1 ml 1 ml
Commercial Isoschizomers: SbfI, Sse8387I
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER1191, #ER1192 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				SdaI (unique)	B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	1X	2X
Buffer SdaI	37°C	0.3	65°C	100	NR	NR	0-20	0-20	NR	20-50

NR: buffer is not recommended, because of high star activity.

Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C), 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 5-fold overdigestion with SdaI, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  never overlaps  never overlaps  never overlaps  never overlaps	- no effect. - no effect. - no effect. - no effect. - no effect.

Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Compatible Ends
Alw21I, Bme1580I, BseSI, Mph1103I, NsiI, PstI, SduI.

Note
Greater than 5-fold overdigestion with SdaI may result in star activity.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer SdaI	NR	20-50

NR: buffer is not recommended, because of high star activity.

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
5	0	1	0	1	0	1	0	0	0	0

Updated balandžio 22, 2008 11:38