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SchI (PleI*)
Available in FastDigest® format

* Unlike PleI, SchI produces DNA fragments with blunt ends 5'-G A G T C N N N N N^-3' 3'-C T C A G N N N N N^-5'		digested lambda DNA 1.4% agarose
#ER1371 Supplied with: 10X Buffer Tango™	1000 u   1 ml
Commercial Isoschizomers: MlyI, (PleI), (PpsI) Enzymes in parentheses have different cleavage specificities.
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER1371 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer Tango™	37°C	0.2	65°C	20-50	50-100	0-20	0-20	100	0-20

Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C), 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 10-fold overdigestion with SchI, approximately 90% of the digested DNA fragments can be ligated and more than 90% of these can be recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  never overlaps  may overlap  never overlaps  may overlap	- no effect. - no effect. - no effect. - no effect. - effect not determined.

Note

• Greater than 10-fold overdigestion with SchI may result in star activity.
• SchI may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid an atypical DNA band pattern, use the 6X Loading Dye & SDS Solution (#R1151) for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer Tango™	100	NR

NR: buffer is not recommended, since enzyme activity is less than 20%.

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
61	10	8	4	4	3	7	6	6	6	4

Updated balandžio 22, 2008 11:38