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ScaI
Available in FastDigest® format

5'-A G T^A C T-3' 3'-T C A^T G A-5'		digested lambda DNA 0.7% agarose
#ER0431 Supplied with: 10X Buffer ScaI	1000 u   1 ml
#ER0432 Supplied with: 10X Buffer ScaI	5000 u   2x1 ml
Commercial Isoschizomers: AssI, BmcAI, ZrmI
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER0431, #ER0432 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				ScaI (unique)	B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	1X	2X
Buffer ScaI	37°C	0.5	80°C(10 u)	100	0-20	0-20	0-20	0-20	0-20	0-20

80°C(10 u) indicates that only small amounts of enzyme (up to 10 units) can be inactivated at 80°C in 20 minutes.

Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with ScaI, more than 80% of the digested DNA fragments can be ligated and more than 90% of these can be recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  never overlaps  never overlaps  never overlaps  may overlap	- no effect. - no effect. - no effect. - no effect. - blocked.

Digestion of Agarose-embedded DNA
Minimum 20 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Double Digestions using Tango™ Buffer

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer ScaI	NR	NR

NR: buffer is not recommended, since enzyme activity is less than 20% or star activity appears at a 5-fold overdigestion (5 units x 1 hour).

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
5	0	0	1	1	1	1	1	1	1	1

Updated balandžio 22, 2008 11:38