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C A T A L O G
 

SatI (Fnu4HI)
Available in FastDigest® format 

5'-G C^N G C-3'
3'-C G N^C G-5' 
digested lambda DNA
1.4% agarose
      
#ER1641
Supplied with:
10X Buffer G
10X Buffer Tango™		 200 u
 
1 ml
1 ml
#ER1642
Supplied with:
10X Buffer G
10X Buffer Tango™		 1000 u
 
1 ml
1 ml
Commercial Isoschizomers:
BisI, Fnu4HI, Fsp4HI, ItaI

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER1641, #ER1642
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 2X
Buffer G 37°C 0.1 65°C 20-50 100 20-50 20-50 50-100 20-50

Storage Buffer
 10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with SatI, more than 60% of the digested DNA fragments can be ligated in a reaction mixture containing 10-30 u of T4 DNA Ligase/1 µg of fragments and 10% PEG. More than 90% of these can be recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 never overlaps
 may overlap
 never overlaps
 never overlaps		 - no effect.
- no effect.
- blocked.
- no effect.
- no effect.

Compatible Ends
GC^(C/G)GC - BcnI, Bme1390I, NciI, ScrFI.
GC^(A/T)GC - Bme1390I, MvaI, ScrFI.

Note
At least two copies of SatI (Fnu4HI) recognition site are required for efficient cleavage.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer G 50-100 20-50

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
380 31 17 42 19 19 20 23 23 19 37

See also General Properties of Restriction Enzymes:

 

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Updated birželio 30, 2009 12:55