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SalI
Available in FastDigest® format

5'-G^T C G A C-3' 3'-C A G C T^G-5'		digested lambda DNA 0.7% agarose
#ER0641 Supplied with: 10X Buffer O 10X Buffer Tango™	1500 u   1 ml 1 ml
#ER0642 Supplied with: 10X Buffer O 10X Buffer Tango™	5x1500 u   2x1 ml 1 ml
#ER0643 Supplied with: 10X Buffer O 10X Buffer Tango™	HC, 7500 u   2x1 ml 1 ml
#ER0645 Supplied with: 10X Buffer O 10X Buffer Tango™	2000 u   1 ml 1 ml
Commercial Isoschizomers: -
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER0641, #ER0642, #ER0643, #ER0645 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl; 50 u/µl, HC

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer O	37°C	0.1	65°C	0-20	0-20	100	20-50	0-20	50-100

Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C), 100 mM KCl, 0.1 mM EDTA, 10 mM 2-mercaptoethanol, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with SalI, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  never overlaps  completely overlaps  never overlaps  never overlaps	- no effect. - no effect. - blocked. - no effect. - no effect.

Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Compatible Ends
AvaI, Eco88I, SmoI, XhoI.

Note
Low salt, high glycerol (>5%) concentrations, pH >8.0 or large excess of the enzyme may result in star activity.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer O	NR	50-100

NR: buffer is not recommended, since enzyme activity is less than 20%.

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
2	0	1	1	1	1	1	1	1	0	1

Updated balandžio 22, 2008 11:39