Home  Contacts  Order  Catalog  Support  Search  Alphabetical Index  Numerical Index  Restriction Enzymes  Modifying Enzymes  PCR, qPCR, RT-PCR & dNTPs  Molecular Cloning  Nucleic Acid Purification  Molecular Labeling & Detection  In vitro Transcription  Electrophoresis Products  Nucleotides  Transfection Reagents  Reagents C A T A L O G

SacI
Available in FastDigest® format

5'-G A G C T^C-3' 3'-C^T C G A G-5'		digested lambda DNA 0.7% agarose
#ER1131 Supplied with: 10X Buffer SacI 10X Buffer Tango™	1200 u   1 ml 1 ml
#ER1132 Supplied with: 10X Buffer SacI 10X Buffer Tango™	5x1200 u   2x1 ml 1 ml
#ER1133 Supplied with: 10X Buffer SacI 10X Buffer Tango™	HC,6000 u   2x1 ml 1 ml
#ER1135 Supplied with: 10X Buffer SacI 10X Buffer Tango™	2000 u   1 ml 1 ml
Commercial Isoschizomers: (Ecl136IIm), (EcoICRI), Psp124BI, SstI Enzymes in parentheses have different cleavage specificities. "m" indicates different sensitivity to methylation.
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER1131, #ER1132, #ER1133, #ER1135 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl; 50 u/µl, HC

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				SacI (unique)	B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	1X	2X
Buffer SacI	37°C	0.2	65°C	100	50-100	20-50	0-20	0-20	50-100	20-50

Storage Buffer
10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with SacI, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  never overlaps  may overlap  never overlaps  may overlap	- no effect. - no effect. - no effect. - no effect. - no effect.

Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Compatible Ends
Alw21I, Eco24I, SduI.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer SacI	50-100	20-50

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
2	0	1	0	1	1	1	1	1	0	0

Updated balandžio 22, 2008 11:39