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PsuI (BstYI)
Available in FastDigest® format

5'-R^G A T C Y-3' 3'-Y C T A G^R-5'		digested lambda DNA 0.7% agarose
#ER1551 Supplied with: 10X Buffer B 10X Buffer Tango™	500 u   1 ml 1 ml
Commercial Isoschizomers: BstX2I, BstYI, MflIm, XhoII "m" indicates different sensitivity to methylation.
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER1551 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer B	37°C	0.5	80°C	100	20-50	0-20	0-20	50-100	0-20

Storage Buffer
10 mM Tris-HCl (pH 7.4 at 25°C), 200 mM NaCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with PsuI, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	completely overlaps  may overlap  may overlap  never overlaps  may overlap	- no effect. - no effect. - no effect. - no effect. - effect not determined.

Note
Low salt, high glycerol (>5%) concentrations, pH >8.0 or large excess of the enzyme may result in star activity.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer B	50-100	NR

NR: buffer is not recommended, since enzyme activity is less than 20%.

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
21	0	3	8	7	7	7	7	7	6	2

Updated gegužės 07, 2008 14:18