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PauI (BssHII) 

5'-G^C G C G C-3'
3'-C G C G C^G-5'

digested lambda DNA
0.7% agarose
       
#ER1091
Supplied with:
10X Buffer R
10X Buffer Tango™		 200 u
 
1 ml
1 ml
#ER1092
Supplied with:
10X Buffer R
10X Buffer Tango™		 1000 u
 
1 ml
1 ml
Commercial Isoschizomers: BsePI, BssHII

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER1091, #ER1092
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 2X
Buffer R 37°C 0.2 80°C 0-20 0-20 100 100 0-20 100

Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with PauI, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 never overlaps
 completely overlaps
 never overlaps
 never overlaps		 - no effect.
- no effect.
- blocked.
- no effect.
- no effect.

Digestion of Agarose-embedded DNA
Minimum 10 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Note
Low salt, high glycerol (>5%) concentrations or large excess of the enzyme may result in star activity.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer R NR 100

NR: buffer is not recommended, since enzyme activity is less than 20%.

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
6 1 0 0 0 0 0 2 2 0 0

See also General Properties of Restriction Enzymes:

 

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Updated kovo 07, 2008 15:04