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PasI 

         A
5'-C C^C T G G G-3'
3'-G G G A C^C C-5'
         T

digested lambda DNA
0.7% agarose
       
#ER1861
 Supplied with:
10X Buffer PasI		 200 u
 
1 ml
Commercial Isoschizomers: -

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER1861
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
PasI
(unique)		 B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 1X 2X
Buffer PasI 55°C 0.2 80°C 100 NR NR NR NR NR NR

NR: buffer is not recommended, because of high star activity.

Storage Buffer
10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 10-fold overdigestion with PasI, more than 90% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 completely overlaps
 never overlaps
 never overlaps
 never overlaps		 - no effect.
- no effect.
- no effect.
- no effect.
- no effect.

Digestion of Agarose-embedded DNA
Minimum 10 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Note

  • Incubation at 37°C results in 30% activity.

  • Greater than 10-fold overdigestion with PasI may result in star activity.

Double Digestions using Tango™ Buffer

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer PasI NR NR

NR: buffer is not recommended, because of high star activity.

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
2 0 0 0 0 0 0 0 0 0 2

See also General Properties of Restriction Enzymes:

 

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Updated vasario 06, 2008 16:06