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C A T A L O G
 

NsbI (FspI)
Available in FastDigest® format

5'-T G C^G C A-3'
3'-A C G^C G T-5'

digested lambda DNA
0.7% agarose
       
#ER1221
Supplied with:
10X Buffer Tango™		 400 u
 
1 ml
#ER1222
Supplied with:
10X Buffer Tango™		 2000 u
 
1 ml
Commercial Isoschizomers:
Acc16I, AviII, FspI

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER1221, #ER1222
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 2X
Buffer Tango™ 37°C 0.1 65°C 20-50 50-100 0-20 20-50 100 20-50

Storage Buffer
10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with NsbI, more than 80% of the digested DNA fragments can be ligated and more than 95% of these can be recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 never overlaps
 completely overlaps
 may overlap
 never overlaps		 - no effect.
- no effect.
- blocked.
- no effect.
- no effect.

Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer Tango™ 100 20-50

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
15 1 1 4 2 2 2 2 2 1 3

See also General Properties of Restriction Enzymes:

 

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Updated balandžio 22, 2008 11:30