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NmuCI (Tsp45I)
Available in FastDigest® format

C 5'-^G T G A C -3' 3'- C A C T G^-5' G		digested lambda DNA 1.4% agarose
#ER1511 Supplied with: 10X Buffer R 10X Buffer Tango™	200 u   1ml 1ml
#ER1512 Supplied with: 10X Buffer R 10X Buffer Tango™	1000 u   1ml 1ml
Commercial Isoschizomers: Tsp45I
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER1511, #ER1512 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer R	37°C	0.1	65°C	0-20	20-50	50-100	100	20-50	50-100

Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C), 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with NmuCI, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  never overlaps  may overlap  never overlaps  may overlap	- no effect. - no effect. - cleavage impaired. - no effect. - effect not determined.

Compatible Ends
Eco91I, MaeIII.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer R	20-50	50-100

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
81	8	8	9	4	4	4	4	4	5	7

Updated balandžio 22, 2008 11:30