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MvaI (BstNI)
Available in FastDigest® format

T 5'-C C^A G G-3' 3'-G G T^C C-5' A		digested lambda DNA 1.4% agarose
#ER0551 Supplied with: 10X Buffer R 10X Buffer Tango™	2000 u   1 ml 1 ml
#ER0552 Supplied with: 10X Buffer R 10X Buffer Tango™	5x2000 u   2x1 ml 1 ml
Commercial Isoschizomers: (AjnI), BptI, BseBI, Bst2UI, BstNI, BstOI, (EcoRIIm), (Psp6I), (PspGIm) Enzymes in parentheses have different cleavage specificities. "m" indicates different sensitivity to methylation.
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER0551, #ER0552 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) rochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer R	37°C	0.1	80°C(10 u)	20-50	20-50	50-100	100	20-50*	100

80°C(10 u) indicates that only small amounts of enzyme (up to 10 units) can be inactivated at 80°C in 20 minutes.
* Star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).

Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C), 400 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 10-fold overdigestion with MvaI, more than 90% of the digested DNA fragments can be ligated in the reaction mixture containing 20-40 u of T4 DNA Ligase/1 µg of fragments and 10% PEG. More than 90% of these can be recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  completely overlaps  never overlaps  never overlaps  never overlaps	- no effect. - no effect. - no effect. - no effect. - no effect.

Compatible Ends
Bme1309I, Fnu4HI, SatI, ScrFI.

Note

• Low salt, high glycerol (>5%) concentrations or large excess of the enzyme may result in star activity.
• Unlike its neoschizomer EcoRII, MvaI does not require multiple copies of recognition site for efficient cleavage.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer R	20-50*	100

* Star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
70	2	7	6	5	5	5	5	5	8	12

Updated balandžio 22, 2008 11:27