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MspI (HpaII)
Available in FastDigest® format

5'-C^C G G-3'
3'-G G C^C-5'

digested lambda DNA
1.4% agarose
     
#ER0541
Supplied with:
10X Buffer Tango™		 3000 u
 
2x1 ml
#ER0542
Supplied with:
10X Buffer Tango™		 5x3000 u
 
2x1 ml
Commercial Isoschizomers:
BsiSI, HapIIm, HpaIIm
"m" indicates different sensitivity to methylation.

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER0541, #ER0542
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 2X
Buffer Tango™ 37°C 0.3 65°C 50-100 50-100 0-20 0-20 100 50-100

Storage Buffer
10 mM potassium phosphate (pH 7.5 at 25°C), 200 mM NaCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with MspI, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 never overlaps
 completely overlaps
 never overlaps
 never overlaps		 - no effect.
- no effect.
- no effect.
- no effect.
- no effect.

Compatible Ends
AccI, AciI, BsaHI, Bsp119I, Bsu15I, ClaI, Hin1I, Hin6I, HinP1I, MaeII, NarI, Psp1406I, SsiI, TaqI, XmiI.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer Tango™ 100 50-100

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
328 5 18 26 13 13 12 13 13 16 34

See also General Properties of Restriction Enzymes:

 

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Updated balandžio 22, 2008 11:28