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MboI
Available in FastDigest® format  

5'-^G A T C -3' 3'- C T A G^-5'		digested lambda DNA (dam-) 1.4% agarose
#ER0811 Supplied with: 10X Buffer R 10X Buffer Tango™	300 u   1 ml 1 ml
#ER0812 Supplied with: 10X Buffer R 10X Buffer Tango™	1500 u   1 ml 1 ml
Commercial Isoschizomers: BfuCIm, Bsp143Im, BssMI, (BstKTI), BstMBI, (DpnI), DpnIIm, Kzo9Im, (MalI), NdeIIm, Sau3AIm Enzymes in parentheses have different cleavage specificities. "m" indicates different sensitivity to methylation.
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER0811, #ER0812 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) rochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer R	37°C	0.1	65°C	50-100	50-100	50-100	100	50-100	100

Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with MboI, more than 95% of the digested DNA dam- fragments can be ligated and recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	completely overlaps  never overlaps  may overlap  never overlaps  may overlap	- blocked. - no effect. - no effect. - no effect. - blocked.

Compatible Ends
BamHI, BclI, BglII, PsuI.

Note
Assayed using lambda DNA (dam-) (#SD0021).

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer R	50-100	100

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
116	0	7	22	15	15	15	15	15	22	15

Updated balandžio 22, 2008 11:29