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LweI (SfaNI)

5'-G C A T C (N)5^-3'
3'-C G T A G (N)9^-5'

digested lambda DNA
1.4% agarose
      
#ER1621
Supplied with:
10X Buffer Tango™		 100 u
 
1 ml
#ER1622
Supplied with:
10X Buffer Tango™		 500 u
 
1 ml
Commercial Isoschizomers: SfaNI

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER1621, #ER1622
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 2X
Buffer Tango™ 37°C 0.2 65°C 0-20 0-20 0-20 20-50 100 20-50

Storage Buffer
 10 mM Tris-HCl (pH 7.5 at 25°C), 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with LweI, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 never overlaps
 may overlap
 never overlaps
 may overlap		 - no effect.
- no effect.
- cleavage impaired.
- no effect.
- effect not determined.

Note
LweI may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid an atypical DNA band pattern, use the 6X Loading Dye & SDS Solution (#R1151) for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer Tango™ 100 20-50

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
169 12 7 22 8 9 4 4 4 17 16

See also General Properties of Restriction Enzymes:

 

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Updated kovo 07, 2008 14:50