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Lsp1109I (BbvI)
Available in FastDigest® format

5'-G C A G C (N)8 ^-3' 3'-C G T C G (N)12^-5'		digested lambda DNA 1.4% agarose
#ER2071 Supplied with: 10X Buffer Lsp1109I 10X Buffer Tango™	200 u   1 ml 1 ml
Commercial Isoschizomers: BbvI, BseXI, BstV1I
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER2071 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
1-3 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				Lsp1109I (unique)	B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	1X	2X
Buffer Lsp1109I	37°C	0.5	65°C	100	0-20	20-50*	50-100*	100	20-50*	20-50*

* Star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).

Storage Buffer
Lsp109I is supplied in: 10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.

Ligation and Recleavage
After 10-fold overdigestion with Lsp1109I, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  never overlaps  may overlap  never overlaps  may overlap	- no effect. - no effect. - no effect. - no effect. - effect not determined.

Note

• Assayed using pBR322 DNA.
• Lsp1109I may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye & SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis.
• Greater than 32-fold overdigestion with Lsp1109I may result in star activity.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer Lsp1109I	20-50*	20-50*

* Star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
199	14	10	21	12	12	12	13	13	7	15

Updated balandžio 22, 2008 11:29